Acrobeles complexus

The Blaxter Lab

Nematode & Neglected Genomics
@ The Blaxter Lab, Institute of Evolutionary Biology, School of Biological Sciences, The University of Edinburgh

University of Edinburgh crest   
barcode worm Molecular Analyses of Diversity
& DNA barcoding

DNA Barcoding

is a molecular technique for discovery and identification of taxa

  • DNA BARCODING We use DNA barcoding as a survey tool for exploring the unknown diversity in ecosystems. DNA barcoding is an approach to specimen identification and biodiversity assessment through the use of DNA sequences. We are developing software tools for anayses of DNA barcode data - jMOTU (and its precursor MOTU_define).


Molecular Operational Taxonomic Units

MOTU is an acronym we invented in the late 1990's to describe the clusters of specimens we were defining based on sequence identity (see here for the original text). A search of online multilingual dictionaries however, revealed that the phoneme has been used for millenia by peoples of the Pacific, where, spookily, it almost means the same thing: to cut off (or define) and an islet (or cluster). Most entertainingly, the word is also used in the context of writing or inscribing, the giving-physical-name-to-things (see below).

The image is of one of the few extant carved wooden panels inscribed with the language of Easter Island.

  the carved writing of Easter Island

motu (definition in Easter Island)

1. to cut; to snap off: motu-á te hau, the fishing line snapped off; to engrave, to inscribe letters or pictures in stone or in wood, like the motu mo rogorogo, inscriptions for recitation in lines called kohau.

2. islet; some names of islets: Motu Motiro Hiva, Sala y Gómez; and around the island: Motu Nui, Motu Iti, Motu Kaokao, Motu Tapu, Motu Marotiri, Motu Kau, Motu Tavake, Motu Tautara, Motu Ko Hepa Ko Maihori, Motu Hava.

see here for our original definition of MOTU, as posted on the www in 1999

see here for jMOTU, our latest tool for MOTU inference from sequence data

 


 

Barcoding Nematodes, Tardigrades and other Meiofauna

We are using molecular methods to perform surveys of nematode and other meiofaunal biodiversity in terrestrial and marine habitats. This work builds from our interest in nematode molecular phylogenetics and an enthusiasm for neglected meiobeasts.

 

what

why

who

the carved writing of Easter Island

The MOTU concept

Using molecular markers to define taxa

Robin Floyd, Eyualem Abebe and Mark Blaxter

barcoding life

MOTU applied to the terrestrial nematodes of Sourhope Farm

As part of the NERC soil biodiversity and ecosystem function programme we are analysing nematode diversity on a test site in southern Scotland

Robin Floyd, Eyualem Abebe and Mark Blaxter

barcoding life

MOTU applied to marine nematodes

We have performed preliminary surveys of marine nematode diversity in Scotland using the MOTU method.

Robin Floyd, Eyualem Abebe and Mark Blaxter

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Nematode Population Genetics

a website collating information on molecular methods for nematode population genetics and diversity studies

Barbara Wimmer and Mark Blaxter

 


Website Highlight
Erpobdella testacea

Larval Erpobdella testacea, a freshwater leech, in their cocoon.
Leeches are annelids: see LumbriBase for analysis of expressed sequence tags from annelids (leech, eathworm and ragworm).

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